Things You Need To Know While Using Fiber photometry
In most straightforward format, fiber photometry will allow is a single photodiode, which is capable of provides recording from many populations of neurons and do not contribute to any spatial information. Fiber photometry cannot be used unless the fluorescent sensors such as dopamine and calcium indicators are first shown at the area of interest. As soon as the sensors are expressed in the area of interest, the optical fiber will then be used to make the sensor excited while detecting the emerging fluorescence.
However, when used in animals which are moving freely, it can be a problem using a ling optical capable to the head of the animal. As a result of having a long rope, there could restriction of the animal to interact while at the same time impend it to movements to area which has got shade or exercise wheels. Increasingly, the cable is capable of causing artifacts in case it’s used with video tracking devices. For example, the software might not recognize the freezing behavior because the intelligent might continue to swaying even when the animal stop. However, if you want to end all these problems, choose to have the components placed on a head stage. Find out the described advantages of using fiber photometry.
Choose to use fiber photometry because it results to reduced weight on the head of the animal, as electrons wot be mounted on its head. As a result, this less amount of heat loss from the animal, because no particles require to be put on the head of the animal. Fiber photometry is much affordable because its simple set up will consist of fiber and fiber amount, simple photoelectrons, bandpass filters as well as a detector, of which all these are inexpensive photodiode. Increasingly, choose to have a fiber photometry, as there are possibilities of rapid rate acquisition which is facilitated by the analogue to digital conversion of photodiodes.
More so, choose to have fiber photometry because its photodiodes have a broader sensitivity. This is because the signals are chosen from a large volume, making it possible for detecting low activity levels. Additionally, with the use of fiber photometry, you’ll be able to customize the fiber into various ways including tethering and painting to get multiple qualities of geometry and optical activities. Also, considering that less amount of fibers are used in fiber photometry, it implies that the technique is less invasive as compared to other ways which use implants. Also, thinner fibers can as well be used, but this would eventually lead to compromise when recording, or else lead to low level of signals.